English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/9525
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 30 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Compaction process of calf thymus DNA by mixed cationic-zwitterionic liposomes: a physicochemical study

Autor Rodríguez-Pulido, Alberto; Aicart, Emilio; Llorca, Óscar ; Junquera, Elena
Fecha de publicación 21-feb-2008
EditorAmerican Chemical Society
Citación J. Phys. Chem. B 112 (7): 2187-2197 (2008)
ResumenThe compaction of calf thymus DNA (CT-DNA) by cationic liposomes constituted by a 1:1 mixture of a cationic lipid, 1,2-distearoyl-3-(trimethylammonio)propane chloride (DSTAP), and a zwitterionic lipid, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE, null net charge at pH = 7.4), has been evaluated in aqueous buffered solution at 298.15 K by means of conductometry, electrophoretic mobility, cryo-TEM, and fluorescence spectroscopy techniques. The results reveal that DSTAP/DOPE liposomes are mostly spherical and unilamelar, with a mean diameter of around 77 ± 20 nm and a positively charged surface with a charge density of σ(ζ) = (21 ± 1) × 10-3 C m-2. When CT-DNA is present, the genosomes DSTAP/DOPE/CT-DNA, formed by means of a surface electrostatic interaction, are generally smaller than the liposomes. Furthermore, they show a tendency to fuse forming cluster-type structures when approaching isoneutrality, which has been determined by the electrochemical methods at around (L/D)(Φ) = 5.6. The analysis of the decrease on the fluorescence emission of the fluorophore ethidium bromide, EtBr, initially intercalated between DNA base pairs, as long as the genosomes are formed has permitted us to confirm the electrostatic character of the DNA−liposome interaction.
Descripción 11 pages, 10 figures.-- PMID: 18220388 [PubMed].-- Supporting Information (Emission fluorescence spectra of EtBr in the presence of DSTAP/DOPE liposomes at different L/D ratios, 3 pages) available at: http://pubs.acs.org/doi/suppl/10.1021/jp7095828/suppl_file/jp7095828-file003.pdf
Versión del editorhttp://dx.doi.org/10.1021/jp7095828
URI http://hdl.handle.net/10261/9525
DOI10.1021/jp7095828
ISSN1520-6106
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Acceso restringido.pdf21,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.