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dc.contributor.authorHeermann, Ralf-
dc.contributor.authorZigann, Katja-
dc.contributor.authorRodríguez Fernández, María-
dc.contributor.authorBanga, Julio R.-
dc.contributor.authorKremling, Andreas-
dc.contributor.authorJung, Kirsten-
dc.identifier.citationPLoS ONE 9(2): e89671 (2014)es_ES
dc.description15 páginas, 4 figuras, 1 tablaes_ES
dc.description.abstractKdpD and KdpE form a histidine kinase/response regulator system that senses K+ limitation and induces the kdpFABC operon, which encodes a high-affinity K+ uptake complex. To define the primary stimulus perceived by KdpD we focused in this study on the dynamics of the Kdp response. Escherichia coli cells were subjected to severe K+ limitation, and all relevant parameters of the Kdp response, i.e., levels of kdpFABC transcripts and KdpFABC proteins, as well as extra- and intracellular K+ concentrations, were quantitatively analysed over time (0 to 180 min). Unexpectedly, induction of kdpFABC was found to follow a non-monotonic time-course. To interpret this unusual behaviour, a mathematical model that adequately captures the dynamics of the Kdp system was established and used for simulations. We found a strong correlation between KdpD/KdpE activation and the intracellular K+ concentration, which is influenced by the uptake of K+ via the KdpFABC complex. Based on these results a model is proposed in which KdpD/KdpE phosphorylation is inversely correlated with the intracellular K+ concentration. To corroborate this hypothesis an isogenic mutant that produces a defective KdpFABC complex, and the trans-complemented mutant that expresses the KtrAB high-affinity K+ uptake system of Vibrio alginolyticus were quantitatively analysed. Experimental data and simulations for the mutants consistently support the tight correlation between KdpD/KdpE activation and the intracellular K+ concentration. This study presents a striking example of the non-intuitive dynamics of a functional unit comprising signalling proteins and a transporter with K+ as mediatores_ES
dc.description.sponsorshipThis work was financially supported by the Deutsche Forschungsgemeinschaft (Exc114/1+2 and JU270/15-1) and the BMBF (SysMO, project 4). MRF and JRB acknowledge financial support from the EU ERASysBio programme and the Spanish MICINN and MINECO (SYSMO grant KOSMOBAC, ref. GEN2006-27747-E/SYS and project MultiScales ref. DPI2011-28112-C04-03; both with partial support from the European Regional Development Fund).es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher’s version-
dc.titleDynamics of an interactive network composed of a bacterial two-component system, a transporter and K+ as mediatores_ES
dc.description.peerreviewedPeer reviewedes_ES
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