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Development of a new bioluminescent mutagenicity assay based on the Ames test

AuthorsGuadaño, Ana; Peña de Torres, Eduardo de la ; González-Coloma, Azucena ; Alvarez, J.F.
KeywordsAmes test
Adenosine triphosphate (ATP)-bioluminiscence
Salmonella typhimurium
Issue Date1999
PublisherOxford University Press
CitationMutagénesis 14 (4): 411-415 (1999)
AbstractA newly developed rapid mutagenicíty assay based on the adenosine triphosphate (ATP)-bioluminescence techníque and the Ames test is described. Salmonella typhimurium strains TA98 and TA100 were exposed in an appropriate liquid medium to the direct mutagens 4-nitroquinoline-N-oxide and methyl methanesulphonate, respectively, and to the indirect mutagen 2-aminoanthracene. Both euxotrophic and prototrophic growth were monitored throughout the incubation period as variations in the intracellular ATP levels by means of the luciferin-luciferase assay. Ater 9-12 h of incubation a dose-response increase in the levels of ATP was readily detected. In order to demonstrate that this increase was due to the growth of revertant bacteria, aliquots from each culture were plated un minimal agar plates. A very good correlation berween the changes in ATP Ievels and the appearance of revertant colonies on the plates was found . Given the rapidity of this method as compared with conventional mutagenicity assays, ít has potential for industrial and environmental applications.Other potential applications are also díscussed.
Description5 páginasy 5 figuras
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