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Single frequency impedimetric immunosensor for atrazine detection

AutorRodríguez, Ángel; Valera, Enrique; Ramón-Azcón, Javier; Sánchez Baeza, Francisco J.; Marco, María Pilar; Castañer, Luis M.
Palabras claveImmunosensors
Impedance spectroscopy
Interdigitate μ-electrodes
Fecha de publicación22-feb-2008
CitaciónSensor and Actuators B Chemical 129(2):921-928 (2007)
ResumenA novel impedimetric immunosensor for the detection of a wide variety of compounds, based on a two coplanar non-passivated interdigitated metallic μ-electrodes and non-labeled immunoreactives, has been designed and developed. A very useful detection method is the impedance spectroscopy sweeping a wide frequency range. This is a powerful, but cumbersome tool to study the sensor performance providing trustworthy results. In this work, it is shown that the exploitation of the changes observed of the impedance at a single frequency provide very good correlation with antibody concentration and hence greatly reduces the signal acquisition and processing complexity. It has thereby a great potential for low cost, low power, fast response and simple use for the in-field or at-line applications. In this work, the application of the method to atrazine detection is described. The results show that the immunosensor signal is a function of the pesticide concentration following a competitive binding relationship, with limits of detection lower than the maximum residue level required by EC for atrazine in wine grapes and other foodstuff products.
Descripción8 pages, 8 figures.-- Available online on Oct 10, 2007.
Versión del editorhttp://dx.doi.org/10.1016/j.snb.2007.10.003
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