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Título

Transferability of molecular markers from major legumes to Lathyrus spp. for their application in mapping and diversity studies

Autor Almeida, Nuno Felipe; Leitao, Susana T.; Caminero, Constantino; Torres, Ana M.; Rubiales, Diego ; Vaz Patto, María Carlota
Palabras clave Microsatellite
Legumes
Lathyrus spp
Resistance gene analogue
Single nucleotide polymorphism
Intron-targeted amplified polymorphic
Cross-genera amplification
Fecha de publicación ene-2014
EditorSpringer
Citación Molecular Biology Reports 41(1): 269-283 (2014)
ResumenLathyrus cicera L. (chickling pea) and L. sativus L. (grass pea) have great potential among grain legumes due to their adaptability to inauspicious environments, high protein content and resistance to serious diseases. Nevertheless, due to its past underused, further activities are required to exploit this potential and to capitalise on the advances in molecular biology that enable improved Lathyrus spp. breeding programmes. In this study we evaluated the transferability of molecular markers developed for closely related legume species to Lathyrus spp. (Medicago truncatula, pea, lentil, faba bean and lupin) and tested the application of those new molecular tools on Lathyrus mapping and diversity studies. Genomic and expressed sequence tag microsatellite, intron-targeted amplified polymorphic, resistance gene analogue and defence-related gene markers were tested. In total 128 (27.7 %) and 132 (28.6 %) molecular markers were successfully cross-amplified, respectively in L. cicera and L. sativus. In total, the efficiency of transferability from genomic microsatellites was 5 %, and from gene-based markers, 55 %. For L. cicera, three cleaved amplified polymorphic sequence markers and one derived cleaved amplified polymorphic sequence marker based on the cross-amplified markers were also developed. Nine of those molecular markers were suitable for mapping in a L. cicera recombinant inbred line population. From the 17 molecular markers tested for diversity analysis, six (35 %) in L. cicera and seven (41 %) in L. sativus were polymorphic and discriminate well all the L. sativus accessions. Additionally, L. cicera accessions were clearly distinguished from L. sativus accessions. This work revealed a high number of transferable molecular markers to be used in current genomic studies in Lathyrus spp. Although their usefulness was higher on diversity studies, they represent the first steps for future comparative mapping involving these species. © 2013 Springer Science+Business Media Dordrecht.
URI http://hdl.handle.net/10261/95058
DOI10.1007/s11033-013-2860-4
Identificadoresdoi: 10.1007/s11033-013-2860-4
issn: 0301-4851
e-issn: 1573-4978
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