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dc.contributor.authorGómez-Gómez, José María-
dc.contributor.authorAmils, Ricardo-
dc.date.accessioned2014-04-02T10:42:01Z-
dc.date.available2014-04-02T10:42:01Z-
dc.date.issued2014-02-25-
dc.identifierhttp://dx.doi.org/10.1186/1756-0500-7-108-
dc.identifier.citationBMC Research Notes 7(1) : 108- (2014)-
dc.identifier.urihttp://hdl.handle.net/10261/94743-
dc.description.abstractAbstract Background Encased in a matrix of extracellular polymeric substances (EPS) composed of flagella, adhesins, amyloid fibers (curli), and exopolysaccharides (cellulose, β-1,6-N-acetyl-D-glucosamine polymer-PGA-, colanic acid), the bacteria Escherichia coli is able to attach to and colonize different types of biotic and abiotic surfaces forming biofilms and colonies of intricate morphological architectures. Many of the biological aspects that underlie the generation and development of these E. coli’s formations are largely poorly understood. Results Here, we report the characterization of a novel E. coli sessile behaviour termed "crowning" due to the bacterial generation of a new 3-D architectural pattern: a corona. This bacterial pattern is formed by joining bush-like multilayered "coronal flares or spikes" arranged in a ring, which self-organize through the growth, self-clumping and massive self-aggregation of cells tightly interacting inside semisolid agar on plastic surfaces. Remarkably, the corona’s formation is developed independently of the adhesiveness of the major components of E. coli’s EPS matrix, the function of chemotaxis sensory system, type 1 pili and the biofilm master regulator CsgD, but its formation is suppressed by flagella-driven motility and glucose. Intriguingly, this glucose effect on the corona development is not mediated by the classical catabolic repression system, the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Thus, corona formation departs from the canonical regulatory transcriptional core that controls biofilm formation in E. coli. Conclusions With this novel "crowning" activity, E. coli expands its repertoire of colonizing collective behaviours to explore, invade and exploit environments whose critical viscosities impede flagella driven-motility.-
dc.description.sponsorshipThe authors express their gratitude to Ilias Tagkopoulos, Jean Marc Ghigo, Paul S. Cohen, Takeshi Mizuno, Hideyuki Suzuki, Regine Hengge and Gisela Storz for kindly providing the strains used in this work and cited in Table 1. We thank Howard Berg and Liyan Ping for providing us the E. coli strain HCB1668, and also to Dra. Paola Bovolenta and her colleagues, from the Centro de Biología Molecular Severo Ochoa (CBMSO), for giving us the opportunity to use their Leika Microscope, as well as to David Hochberg by his critical reading of manuscript. This work was partially supported by grant CGL2012-34020.-
dc.publisherBioMed Central-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleCrowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona-
dc.typeartículo-
dc.date.updated2014-04-02T10:42:01Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066en-
dc.rights.holderJosé María Gómez-Gómez et al.; licensee BioMed Central Ltd.-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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