English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/94743
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona

AuthorsGómez-Gómez, José María; Amils, Ricardo
Issue Date25-Feb-2014
PublisherBioMed Central
CitationBMC Research Notes 7(1) : 108- (2014)
AbstractAbstract Background Encased in a matrix of extracellular polymeric substances (EPS) composed of flagella, adhesins, amyloid fibers (curli), and exopolysaccharides (cellulose, β-1,6-N-acetyl-D-glucosamine polymer-PGA-, colanic acid), the bacteria Escherichia coli is able to attach to and colonize different types of biotic and abiotic surfaces forming biofilms and colonies of intricate morphological architectures. Many of the biological aspects that underlie the generation and development of these E. coli’s formations are largely poorly understood. Results Here, we report the characterization of a novel E. coli sessile behaviour termed "crowning" due to the bacterial generation of a new 3-D architectural pattern: a corona. This bacterial pattern is formed by joining bush-like multilayered "coronal flares or spikes" arranged in a ring, which self-organize through the growth, self-clumping and massive self-aggregation of cells tightly interacting inside semisolid agar on plastic surfaces. Remarkably, the corona’s formation is developed independently of the adhesiveness of the major components of E. coli’s EPS matrix, the function of chemotaxis sensory system, type 1 pili and the biofilm master regulator CsgD, but its formation is suppressed by flagella-driven motility and glucose. Intriguingly, this glucose effect on the corona development is not mediated by the classical catabolic repression system, the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Thus, corona formation departs from the canonical regulatory transcriptional core that controls biofilm formation in E. coli. Conclusions With this novel "crowning" activity, E. coli expands its repertoire of colonizing collective behaviours to explore, invade and exploit environments whose critical viscosities impede flagella driven-motility.
URIhttp://hdl.handle.net/10261/94743
Identifiershttp://dx.doi.org/10.1186/1756-0500-7-108
Appears in Collections:(CAB) Artículos
(CBM) Artículos
Files in This Item:
File Description SizeFormat 
1756-0500-7-108.xml79,81 kBXMLView/Open
1756-0500-7-108-S1.JPEG590,18 kBJPEGThumbnail
View/Open
1756-0500-7-108.pdf3,94 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.