English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/94742
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A single mutation in the GSTe2 gene allows tracking of metabolically-based insecticide resistance in a major malaria vector

AuthorsRiveron, Jacob M.; Yunta, C. ; Ibrahim, Sulaiman S.; Djouaka, Rousseau; Irving, Helen; Menze, Benjamin D.; Ismail, Hanafy M.; Hemingway, Janet; Ranson, Hilary; Albert, Armando ; Wondji, Charles S.
Issue Date25-Feb-2014
PublisherBioMed Central
CitationGenome Biology 15(2) : R27 (2014)
Abstract[Background] Metabolic resistance to insecticides is the biggest threat to the continued effectiveness of malaria vector control. However, its underlying molecular basis, crucial for successful resistance management, remains poorly characterized.
[Results] Here, we demonstrate that the single amino acid change L119F in an upregulated glutathione S-transferase gene, GSTe2, confers high levels of metabolic resistance to DDT in the malaria vector Anopheles funestus. Genome-wide transcription analysis revealed that GSTe2 was the most over-expressed detoxification gene in DDT and permethrin-resistant mosquitoes from Benin. Transgenic expression of GSTe2 in Drosophila melanogaster demonstrated that over-transcription of this gene alone confers DDT resistance and cross-resistance to pyrethroids. Analysis of GSTe2 polymorphism established that the point mutation is tightly associated with metabolic resistance to DDT and its geographical distribution strongly correlates with DDT resistance patterns across Africa. Functional characterization of recombinant GSTe2 further supports the role of the L119F mutation, with the resistant allele being more efficient at metabolizing DDT than the susceptible one. Importantly, we also show that GSTe2 directly metabolizes the pyrethroid permethrin. Structural analysis reveals that the mutation confers resistance by enlarging the GSTe2 DDT-binding cavity, leading to increased DDT access and metabolism. Furthermore, we show that GSTe2 is under strong directional selection in resistant populations, and a restriction of gene flow is observed between African regions, enabling the prediction of the future spread of this resistance.
[Conclusions] This first DNA-based metabolic resistance marker in mosquitoes provides an essential tool to track the evolution of resistance and to design suitable resistance management strategies.
Publisher version (URL)http://dx.doi.org/10.1186/gb-2014-15-2-r27
Appears in Collections:(IQFR) Artículos
Files in This Item:
File Description SizeFormat 
gb-2014-15-2-r27.xml166,9 kBXMLView/Open
GB-2014-15-2-R27-S4.PDF377,32 kBAdobe PDFThumbnail
GB-2014-15-2-R27-S13.DOC33 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S9.DOC677,27 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S1.DOC145,93 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S8.DOC64,89 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S15.DOC49 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S11.DOC806,84 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S5.DOC163,06 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S6.DOC170,9 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S3.DOC27 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S2.DOC86 kBMicrosoft WordView/Open
gb-2014-15-2-r27.pdf2,79 MBAdobe PDFThumbnail
GB-2014-15-2-R27-S14.DOC27,5 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S10.DOC33 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S7.DOC38,5 kBMicrosoft WordView/Open
GB-2014-15-2-R27-S12.DOC360,79 kBMicrosoft WordView/Open
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.