English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/9465
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Oligomerization of the influenza virus polymerase complex in vivo

AutorJorba, Núria; Area, Estela; Ortín, Juan
Palabras claveInfluenza A virus
Polymerase complex
Heterotrimer
Tandem affinity purification (TAP)
Higher-order oligomers
Fecha de publicaciónfeb-2008
EditorSociety for General Microbiology
CitaciónJournal of General Virology 89: 520-524 (2008)
ResumenThe influenza virus polymerase is a heterotrimer formed by the PB1, PB2 and PA subunits and is responsible for virus transcription and replication. We have expressed the virus polymerase complex by co-transfection of the subunit cDNAs, one of which was tandem affinity purification (TAP)-tagged, into human cells. The intracellular polymerase complexes were purified by the TAP approach, involving two affinity chromatography steps, IgG–Sepharose and calmodulin–agarose. Gel-filtration analysis indicated that, although most of the purified polymerase behaved as a heterotrimer, a significant proportion of the purified material migrated as polymerase dimers, trimers and higher oligomers. Co-purification of polymerase complexes alternatively tagged in the same subunit confirmed that the polymerase complex might form oligomers intracellularly. The implications of this observation for virus infection are discussed.
Descripción5 pages.-- PMID: 18198383 [PubMed].
Versión del editorhttp://dx.doi.org/10.1099/vir.0.83387-0
URIhttp://hdl.handle.net/10261/9465
DOI10.1099/vir.0.83387-0
ISSN0022-1317
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.