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Título

The contribution of epistasis to the architecture of fitness in an RNA virus

Autor Sanjuán, Rafael; Moya-Simarro, Andrés; Elena, Santiago F.
Palabras clave Epistasis
Mutations
Evolutionary biology
Interactions among genome components
RNA viruses
Fitness
Fecha de publicación 18-oct-2004
EditorNational Academy of Sciences (U.S.)
Citación Proc. Natl. Acad. Sci. USA 101(43): 15376-15379 (2004)
ResumenThe tendency for genetic architectures to exhibit epistasis among mutations plays a central role in the modern synthesis of evolutionary biology and in theoretical descriptions of many evolutionary processes. Nevertheless, few studies unquestionably show whether, and how, mutations typically interact. Beneficial mutations are especially difficult to identify because of their scarcity. Consequently, epistasis among pairs of this important class of mutations has, to our knowledge, never before been explored. Interactions among genome components should be of special relevance in compacted genomes such as those of RNA viruses. To tackle these issues, we first generated 47 genotypes of vesicular stomatitis virus carrying pairs of nucleotide substitution mutations whose separated and combined deleterious effects on fitness were determined. Several pairs exhibited significant interactions for fitness, including antagonistic and synergistic epistasis. Synthetic lethals represented 50% of the latter. In a second set of experiments, 15 genotypes carrying pairs of beneficial mutations were also created. In this case, all significant interactions were antagonistic. Our results show that the architecture of the fitness depends on complex interactions among genome components.
Descripción 4 pages, 2 figures.-- PMID: 15492220 [PubMed].-- PMCID: PMC524436.-- Additional information (Suppl. table S1: Relevant information about each single- and double-nucleotide substitution mutant created) available at: http://www.pnas.org/content/101/43/15376/suppl/DC1
Versión del editorhttp://dx.doi.org/10.1073/pnas.0404125101
URI http://hdl.handle.net/10261/9415
DOI10.1073/pnas.0404125101
ISSN0027-8424
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