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Título

Relative Contributions of Lipooligosaccharide Inner and Outer Core Modifications to Nontypeable Haemophilus influenzae Pathogenesis

Autor Morey, Pau; Viadas, Cristina ; Euba, Begoña ; Hood, Derek W.; Barberán, Montserrat; Gil Puig, Carmen; Grilló, María Jesús ; Bengoechea, José Antonio ; Garmendia, Juncal
Fecha de publicación nov-2013
EditorAmerican Society for Microbiology
Citación Infection and Immunity 81(11): 4100-4111 (2013)
ResumenNontypeable Haemophilus influenzae (NTHi) is a frequent commensal of the human nasopharynx that causes opportunistic infection in immunocompromised individuals. Existing evidence associates lipooligosaccharide (LOS) with disease, but the specific and relative contributions of NTHi LOS modifications to virulence properties of the bacterium have not been comprehensively addressed. Using NTHi strain 375, an isolate for which the detailed LOS structure has been determined, we compared systematically a set of isogenic mutant strains expressing sequentially truncated LOS. The relative contributions of 2-keto-3-deoxyoctulosonic acid, the triheptose inner core, oligosaccharide extensions on heptoses I and III, phosphorylcholine, digalactose, and sialic acid to NTHi resistance to antimicrobial peptides (AMP), self-aggregation, biofilm formation, cultured human respiratory epithelial infection, and murine pulmonary infection were assessed. We show that opsX, lgtF, lpsA, lic1, and lic2A contribute to bacterial resistance to AMP; lic1 is related to NTHi self-aggregation; lgtF, lic1, and siaB are involved in biofilm growth; opsX and lgtF participate in epithelial infection; and opsX, lgtF, and lpsA contribute to lung infection. Depending on the phenotype, the involvement of these LOS modifications occurs at different extents, independently or having an additive effect in combination. We discuss the relative contribution of LOS epitopes to NTHi virulence and frame a range of pathogenic traits in the context of infection.
Descripción Supplemental material for this article may be found at http://dx.doi.org/10.1128/IAI.00492-13
Versión del editorhttp://dx.doi.org/10.1128/IAI.00492-13
URI http://hdl.handle.net/10261/94108
DOI10.1128/IAI.00492-13
ISSN0019-9567
E-ISSN1098-5522
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