Please use this identifier to cite or link to this item:
|SHARE CORE BASE|
|Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE|
Septins localize to microtubules during nutritional limitation in Saccharomyces cerevisiae
|Authors:||Pablo Hernando, M.ª Evangelina CSIC; Arnáiz-Pita, Yolanda CSIC; Rey Iglesias, Francisco del; Vázquez de Aldana, Carlos R. CSIC ORCID||Keywords:||Saccharomyces cerevisiae
Vegetative septins Cdc10 and Cdc11
Change in localization
Sporulation-specific septins Spr3 and Spr28
|Issue Date:||1-Oct-2008||Publisher:||BioMed Central||Citation:||BMC Cell Biology 9: 55 (2008)||Abstract:||[Background]: In Saccharomyces cerevisiae, nutrient limitation stimulates diploid cells to undergo DNA replication and meiosis, followed by the formation of four haploid spores. Septins are a family of proteins that assemble a ring structure at the mother-daughter neck during vegetative growth, where they control cytokinesis. In sporulating cells, the septin ring disassembles and septins relocalize to the prospore membrane.
[Results]: Here, we demonstrate that nutrient limitation triggers a change in the localization of at least two vegetative septins (Cdc10 and Cdc11) from the bud neck to the microtubules. The association of Cdc10 and Cdc11 with microtubules persists into meiosis, and they are found associated with the meiotic spindle until the end of meiosis II. In addition, the meiosis-specific septin Spr28 displays similar behavior, suggesting that this is a common feature of septins. Septin association to microtubules is a consequence of the nutrient limitation signal, since it is also observed when haploid cells are incubated in sporulation medium and when haploid or diploid cells are grown in medium containing non-fermentable carbon sources. Moreover, during meiosis II, when the nascent prospore membrane is formed, septins moved from the microtubules to this membrane. Proper organization of the septins on the membrane requires the sporulation-specific septins Spr3 and Spr28.
[Conclusion]: Nutrient limitation in S. cerevisiae triggers the sporulation process, but it also induces the disassembly of the septin bud neck ring and relocalization of the septin subunits to the nucleus. Septins remain associated with microtubules during the meiotic divisions and later, during spore morphogenesis, they are detected associated to the nascent prospore membranes surrounding each nuclear lobe. Septin association to microtubules also occurs during growth in non-fermentable carbon sources.
|Description:||14 pages, 7 figures.-- This is an Open Access article distributed under the terms of the Creative Commons Attribution License.-- et al.||Publisher version (URL):||http://dx.doi.org/10.1186/1471-2121-9-55||URI:||http://hdl.handle.net/10261/9403||DOI:||10.1186/1471-2121-9-55||ISSN:||1471-2121|
|Appears in Collections:||(IMB) Artículos|
Show full item record
Files in This Item:
Review this work
checked on Jan 15, 2022
checked on Jan 12, 2022
WEB OF SCIENCETM
checked on Jan 7, 2022
checked on Jan 16, 2022
checked on Jan 16, 2022
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.