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Título

Diversity of the dominant marine nano- and picoeukaryotes – the single cell perspective from the Tara Oceans Expedition

Autor Sieracki, Michael E.; Stepanauskas, R.; Yoon, S.-C.; Massana, Ramon ; Poulton, N.J.; Logares, Ramiro
Fecha de publicación 20-feb-2012
Citación 2012 Ocean Sciences Meeting. Abstract book: 424-425 (2012)
ResumenMarine eukaryotes smaller than 20 µm are highly diverse and not well described, yet they are involved in many key ecosystem functions in the ocean. Diversity surveys based on bulk DNA methods may have distorted our view of the community structure due to the over-representation by types with multiple copies of the SSU rRNA genes. High-throughput sequencing of the SSU rRNA genes from single sorted cells yields a more accurate picture of the diversity of the dominant unicellular eukaryotes. We present here our results from surface waters of the Mediterranean Sea and Indian Ocean, as part of the Tara Oceans Expedition, a circum-global oceanographic survey of plankton diversity. Phototrophs, identified by autofluorescence, are generally less diverse than heterotrophs, and dominated by Prasinophytes, Chrysophytes, and Prymnesiophytes. The heterotrophic cells have more unknown representatives. The heterotrophic marine stramenopiles of the MAST groups are ubiquitous and widely dominant. Single amplified genomes from these cells are a valuable resource for genomic sequencing and studies of the diversity and ecological functions of these important microorganisms
Descripción 2012 Ocean Sciences Meeting, 20-24 February 2012, Salt Lake City, Utah, USA
Versión del editorhttp://www.sgmeet.com/osm2012/
URI http://hdl.handle.net/10261/94007
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