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dc.contributor.authorEsteban, Ana-
dc.contributor.authorDíaz, Margarita-
dc.contributor.authorYepes, Ana-
dc.contributor.authorSantamaría, Ramón I.-
dc.identifier.citationBMC Microbiology 2008, 8:201en_US
dc.description12 pages, 4 figures.-- PMID: 19019225 [PubMed].en_US
dc.description.abstract[Background] PstS is a phosphate-binding lipoprotein that is part of the high-affinity phosphate transport system. Streptomyces lividans accumulates high amounts of the PstS protein in the supernatant of liquid cultures grown in the presence of different carbon sources, such as fructose or mannose, but not in the presence of glucose or in basal complex medium.en_US
dc.description.abstract[Results] Functionality experiments revealed that this extracellular PstS protein does not have the capacity to capture phosphate and transfer it to the cell. Regulation of the pstS promoter was studied with Northern blot experiments, and protein levels were detected by Western blot analysis. We observed that the pstS gene was expressed in cultures containing glucose or fructose, but not in complex basal medium. Northern blot analyses revealed that the pst operon (pstSCAB) was transcribed as a whole, although higher transcript levels of pstS relative to those of the other genes of the operon (pstC, pstA and pstB) were observed. Deletion of the -329/-144 fragment of the pstS promoter, including eight degenerated repeats of a sequence of 12 nucleotides, resulted in a two-fold increase in the expression of this promoter, suggesting a regulatory role for this region. Additionally, deletion of the fragment corresponding to the Pho boxes recognized by the PhoP regulator (from nucleotide -141 to -113) resulted in constitutive pstS expression that was independent of this regulator. Thus, the PhoP-independent expression of the pstS gene makes this system different from all those studied previously.en_US
dc.description.abstract[Conclusion] 1.- In S. lividans, only the PstS protein bound to the cell has the capacity to bind phosphate and transfer it there, whereas the PstS form accumulated in the supernatant lacks this capacity. 2.- The stretch of eight degenerated repeats present in the pstS promoter may act as a binding site for a repressor. 3.- There is a basal expression of the pstS gene that is not controlled by the main regulator: PhoP.en_US
dc.description.sponsorshipAE was the recipient of a Fellowship from the CSIC. AY was the recipient of a Fellowship from the Junta de Castilla y León. This research has been supported by Grants from the Ministerio de Ciencia e Innovación (BFU2005-06392 and BFU2006-13668) to RIS.en_US
dc.format.extent737408 bytes-
dc.publisherBioMed Centralen_US
dc.relation.isversionofPublisher’s version-
dc.subjectPstS lipoproteinen_US
dc.subjectStreptomyces lividansen_US
dc.subjectPhosphate transport systemen_US
dc.subjectCarbon sourcesen_US
dc.subjectPhoP regulatoren_US
dc.titleExpression of the pstS gene of Streptomyces lividans is regulated by the carbon source and is partially independent of the PhoP regulatoren_US
dc.description.peerreviewedPeer revieweden_US
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