English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/93656
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 9 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Biomimetic architectures for the impedimetric discrimination of influenza virus phenotypes

Autor Wicklein, Bernd ; Martín del Burgo, M. Ángeles; Yuste, Miriam ; Carregal-Romero, Ester; Llobera, Andreu; Darder, Margarita ; Aranda, Pilar ; Ortín, Juan; Real, Gustavo del; Fernández-Sánchez, César; Ruiz-Hitzky, Eduardo
Palabras clave Influenza A virus
Hybrid bilayers
Impedimetric detectors
Biomimetic receptors
Phenotype discrimination
Fecha de publicación 2013
EditorWiley-VCH
Citación Advanced Functional Materials 23(2): 254-262 (2013)
ResumenRapid discrimination of avian vs. human phenotypes of emerging influenza A virus isolates with pandemic potential is an important issue in pathogenesis and epidemiology studies of the infection. In this work, functional architectures are tailored on the surface of a gold electrode, introducing receptor molecules as a sensing entity that mimics those found in the membrane of target cells of the influenza A virus and with the aim of developing an impedimetric-based detector for influenza A virus phenotyping. In a bottom-up approach, the artificial receptors are built by sequential assembly of a 1-octanethiol/octyl- galactoside hybrid bilayer, followed by an enzyme-mediated functionalization of the terminal galactoside groups with sialic acid molecules. The detection mechanism relies hence on the specific affinity between the sialic acid-galactose receptor moieties anchored on the modified electrode surface and the hemagglutinin (HA) viral surface protein. By using the appropriate type of sialyltransferase enzyme, sialylation of galactose residues is made through α-2,3 or α-2,6 linkages. This permits the envisaged impedimetric detector to discriminate rapidly between avian vs. human strains of influenza A virus with the absence of elaborate sample preparation steps. In contrast to immunosensors based on antibodies as bioreceptor, the sialylated modified gold electrode is also able to distinguish among influenza phenotypes, which could make the here presented detector a reagentless, label-free diagnostic device for influenza phenotyping. Biomimetic architectures for the impedimetric discrimination of influenza virus phenotypes are tailored by a self-assembly process on gold substrates. Incorporated sialic acid molecules as sensing entities mimic receptors found on target cells of the influenza A virus. The resulting detector rapidly discriminates between avian and human strains of influenza A virus, acting as a reagentless, label-free diagnostic device for influenza phenotyping, which is important in surveillance and prediction of pandemics. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
URI http://hdl.handle.net/10261/93656
DOI10.1002/adfm.201200377
Identificadoresdoi: 10.1002/adfm.201200377
issn: 1616-301X
e-issn: 1616-3028
Aparece en las colecciones: (IMB-CNM) Artículos
(ICMM) Artículos
(CNB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.