English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/93638
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Seasonal Patterns in Proteorhodopsin Gene Dynamics in the Arctic Ocean

AuthorsNguyen, D.; Maranger, R.; Balagué, Vanessa ; Coll-Lladó, Montserrat ; Fernández-Gómez, Beatriz ; Lovejoy, Connie; Pedrós-Alió, Carlos
Issue DateApr-2012
CitationIPY2012 Conference Montréal (2012)
AbstractPhotoheterotrophic microbes have the capacity to use both light and organic matter as an energy source and challenge our classical understanding of the marine C cycle. The proteorhodopsin gene (PR), encoding a membrane-bound retinal, is one of the functional genes that enable some bacteria to harvest light's energy. However, while PR gene has been reported from many oceanic regions, seasonal patterns of PR gene presence and expression remain poorly described, especially in polar oceans. During the Circumpolar Flaw Lead (IPY-CFL) study, we had the opportunity to investigate the annual seasonal pattern of PR gene presence and expression in the Amundsen Gulf of the Arctic Ocean. Surface seawater samples for microbial DNA and RNA were collected weekly from the Amundsen Gulf (November 2007 to July 2008). A subset of these samples was then chosen for targeted PR gene amplification using degenerate primers. Given the contrasting light-dark period in the Arctic, we expected PR to not be expressed during periods of ice cover and present throughout the open water season. PR was present in the genomic DNA of all of the samples tested. However the expression, inferred from the successful amplification of the PR gene from the RNA sample, coincided with the melt season and the return of sunlight only. Furthermore, there was an abrupt seasonal change in PR dynamics in the Arctic, which was mirrored by changes in bacterial diversity estimated using 16S rRNA gene amplicon visualized via denaturing gradient gel electrophoresis (DGGE). The DGGE indicated a rapid change in bacterial communities at ice melt. Light availability was the best indicator of PR dynamics and microbial community structure in the Arctic. Our data adds considerably to the known distribution and expression of the PR gene in marine bacterial communities, and the increased understanding of the timing and occurrence of the PR is a first step towards the integration of photoheterotrophs into Carbon cycle models in the Arctic Ocean
DescriptionIPY2012 Conference Montréal. From Knowledge to Action, 22-27 April 2012, Montréal, Canada
Publisher version (URL)http://132.246.11.198/2012-ipy/Abstracts_On_the_Web/authors.html
URIhttp://hdl.handle.net/10261/93638
Appears in Collections:(ICM) Comunicaciones congresos
Files in This Item:
There are no files associated with this item.
Show full item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.