English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/93508
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Fate of artificial microRNA-mediated resistance to plant viruses in mixed infections

AuthorsElena, Santiago F. ; Martínez, Fernando ; Daròs Arnau, José Antonio
KeywordsArabidopsis
Coinfection
Disease Resistance
DNA, Complementary
Gene
Expression
Host-Pathogen Interactions
MicroRNAs
Plant Diseases
Plant Leaves
Issue DateAug-2013
PublisherAmerican Phytopathological Society
CitationPhytopathology
AbstractArtificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically downregulate RNAs that contain complementary sequences. Transgenic plants expressing high levels of one or more amiRNAs targeting particular sequences in the genomes of some RNA viruses have shown specific resistance to the corresponding virus. This is the case of the Arabidopsis thaliana transgenic line 12-4 expressing a high level of the amiR159-HCPro targeting 21 nucleotides in the Turnip mosaic virus (TuMV) (family Potyviridae) cistron coding for the viral RNA-silencing suppressor HCPro that is highly resistant to TuMV infection. In this study, we explored the fate of this resistance when the A. thaliana 12-4 plants are challenged with a second virus in addition to TuMV. The A. thaliana 12-4 plants maintained the resistance to TuMV when this virus was co-inoculated with Tobacco mosaic virus, Tobacco rattle virus (TRV), Cucumber mosaic virus (CMV), Turnip yellow mosaic virus, Cauliflower mosaic virus (CaMV), Lettuce mosaic virus, or Plum pox virus. However, when the plants were preinfected with these viruses, TuMV was able to co-infect 12-4 plants preinfected with TRV, CaMV, and, particularly, CMV. Therefore, preinfection by another virus jeopardizes the amiRNA-mediated resistance to TuMV. © 2013 The American Phytopathological Society.
Publisher version (URL)http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-09-12-0233-R
URIhttp://hdl.handle.net/10261/93508
ISSN0031-949X
Appears in Collections:(IBMCP) Artículos
Files in This Item:
File Description SizeFormat 
Martinezetal Phytopathology.pdf692,41 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.