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Brush border structure defects in I-plastin deficient mice

AuthorsGrimm-Günter,; Ramos, Sonia ; Smyth, N.; Revenu, C; Robine, S; Raposo, G.; Rivero , F
Issue Date2006
CitationEuropean Journal of Cell Biology 85: 22 (2006)
AbstractPlastin (or fi mbrin) is an actin-bundling protein consisting of two actin-binding domains in tandem preceded by two calcium-binding EF-hands. In mammals three different isoforms are expressed in a cell-type specifi c manner. Iplastin is specifi cally expressed in the small intestine,colon and kidney, where it localizes in microvilli, specialized surface structures with highly ordered microfi lament bundles. We have generated knockout mice lacking I-plastin. I-plastin defi cient mice showed no overt phenotype at the whole animal level: growth rate, reproductive rate and litter size were normal. Antibodies specifi c for T- and L-plastin were used to demonstrate that lacking of I-plastin is not compensated by another plastin isoform. Transmission electron microscopy studies revealed morphological differences between wildtype and I-plastin defi cient mice in the microvilli. Brush border preparations of knockout mice are sensitive and seem to degrade easily. In immunofl uorescence studies of intestinal mucosa cryosections decreased actin staining of the apical membrane was observed. Another abundant actin cross-linker of the brush border, villin, was found to be absent from the apical membrane. I-plastin seems to be an important regulator of the morphological structure and stability of microvilli.
Identifiersdoi: 10.1016/j.ej...006.02.011
issn: 0171-9335
Appears in Collections:(IF) Artículos
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