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Surgical approach for local treatment strategies of noise induced hearing loss

Autor Sistiaga, J. A.; Sanz, Lorena ; Murillo-Cuesta, Silvia ; Martínez-Vega, Raquel ; Avendaño, Carlos; Varela-Nieto, Isabel ; Rivera, Teresa
Fecha de publicación 2012
Citación IEB (2012)
Resumen[Introduction and Objective]: Local delivery of new drugs into the middle ear represents a valid therapeutic alternative for cochlear damage. However, this strategy requires an innocuous surgical approach and efficient drug delivery systems. The objective of this study is to determine the feasibility and safety of a model of local treatment for noise induced hearing loss in mice, based on the ventral surgical approach to the middle ear and the use of a soaked gelfoam. [Materials and Methods]: 34 two month-old male CBA/CaOlaHsd mice were evaluated with auditory brainstem responses (ABR) before surgery and divided in 2 groups: SHAM (n=20) and noise-exposed (violet swept sine noise, 105 dB SPL for 30 minutes 24h before surgery, n=14). Surgical process consisted in a transcervical approach to the middle ear and a bullostomy, in order to reach the round window niche, where a salinesoaked gelfoam was placed. ABR measurements were repeated 1, 14 and 30 days after surgery. Morphological changes were evaluated with histology and stereological hair cell quantification. [Results]: Baseline ABR thresholds were in the normal hearing range, as expected for this strain. No statistically significant threshold shifts were observed in the SHAM group after surgery. Accordingly, mice from control group presented a normal cytoarchitecture and a homogeneous distribution of hair cells along the cochlea. Mice exposed to noise showed an evident threshold shift (40-50 dB SPL) and altered cochlear morphology, with disruption of stereocilia and loss hair cells, mainly OHC, especially in the basal turn of the cochlea. [Conclusion]: Here we present an easy-to-perform local treatment model with no evidence of functional or morphological damage to the cochlea. This strategy could be used to deliver hydrophilic drugs to the inner ear, although further studies are needed to optimize it.
Descripción Comunicación presentada al 49th Workshop on Inner Ear Biology celebrada en Alemania del 30 de septiembre al 2 de octubre de 2012.
URI http://hdl.handle.net/10261/93286
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