English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/93035
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

c-Myc inhibits CD11a and CD11c leukocyte integrin promoters

AuthorsLópez-Rodríguez, Cristina; Delgado, M. Dolores ; Puig-Kröger, Amaya ; Nueda, Arsenio; Muñoz, Eduardo; León, Javier ; Bernabéu, Carmelo ; Corbí, Angel L.
KeywordsCD11c promoter
Integrin
c-Myc
Transcription
Issue Date2000
PublisherWiley-VCH
CitationEuropean Journal of Immunology 30(9): 2465-2471 (2000)
AbstractThe c-Myc transcription factor is an important regulator of cell growth and differentiation, and its gene repression ability seems to play a key role in Myc-mediated cellular transformation. Since Myc overexpression has been associated with reduced expression of β1 and β2 integrins, we have investigated the role of c-Myc on CD11a and CD11c transcription. c-Myc inhibited CD11a and CD11c integrin promoter activity in co-transfection experiments, and similar repression was obtained in cells where c-Myc expression (KmycB) or activity (Rat-1 c-MycER(TM)) is inducible. The c-Myc repression on the CD11c promoter was independent of the USF-binding site (USF-150), other putative Myc-binding elements, or the integrity of the initiator (Inr)-like sequence present at the major transcriptional start site. Analysis of deletion and mutant promoter constructs revealed that, in the absense of additional upstream cis-acting elements, an AP-1-binding site at -60 (AP1-60) is required for c-Myc repressor activity. The c-Myc repressor activity on both integrin promoters was abrogated by deletion of c-Myc residues 106-143, a domain involved in Inr-dependent transcriptional repression. These results demonstrate a direct effect of c-Myc on integrin gene transcription and suggest the existence of a c-Myc-dependent mechanism for coupling leukocyte integrin expression to the cell proliferative state.
URIhttp://hdl.handle.net/10261/93035
DOI10.1002/1521-4141(200009)30:9<2465::AID-IMMU2465>3.0.CO;2-0
Identifiersissn: 0014-2980
e-issn: 1521-4141
Appears in Collections:(CIB) Artículos
(IBBTEC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.