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Effects of n-butanol on barley microspore embryogenesis

AuthorsCastillo Alonso, Ana María CSIC ORCID ; Nielsen, N. H.; Jensen, A.; Vallés Brau, María Pilar CSIC ORCID
KeywordsAnther culture
Doubled haploid
Microspore cultures
Issue DateJun-2014
CitationCastillo AM, Nielsen NH, Jensen A, Vallés MP. Effects of n-butanol on barley microspore embryogenesis. Plant Cell, Tissue and Organ Culture 117 (3): 411-418 (2014)
AbstractDoubled haploid (DH) production is an efficient tool in barley breeding, but efficiency of DH methods is not consistent. Hence, the aim of this study was to study the effect of n-butanol application on DH barley plant production efficiency. Five elite cultivars of barley and thirteen breeding crosses with different microspore embryogenesis capacities were selected for n-butanol application in anther and isolated microspore cultures. Application of 0.1 % n-butanol after a mannitol stress treatment in anther culture significantly increased the number of embryos (up to almost twice) and green plants (from 1.7 to 3 times) in three low-responding cultivars: Albacete, Astoria and Majestic. No significant differences on microspore embryogenesis efficiency were observed in medium and high responding cultivars. The application of n-butanol treatment to isolated microspores from cold treated spikes in thirteen spring breeding crosses with a low or very low androgenetic response did not have a significant effect on the overall number of green plants. Nevertheless, an increase in the number of green plants was observed when 0.2 % n-butanol was applied in four out of seven low-responding crosses. Therefore, application of n-butanol could be routinely applied to anther cultures using mannitol treatment, in low-responding material. However, further studies are needed to determine optimal conditions in protocols using cold treatment and isolated microspore cultures.
Description16 Pag., 2 Fig., 2 Tabl. Available online 19 February 2014. The definitive version is available at: http://link.springer.com/article/10.1007/s11240-014-0451-2
Publisher version (URL)http://dx.doi.org/10.1007/s11240-014-0451-2
Appears in Collections:(EEAD) Artículos
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CastilloA_PlantCellTissOrg_2014_Figure2.pdfFigura 216,22 kBAdobe PDFThumbnail
CastilloA_PlantCellTissOrg_2014_Table1.pdfTabla 117,48 kBAdobe PDFThumbnail
CastilloA_PlantCellTissOrg_2014_Table2.pdfTabla 212,04 kBAdobe PDFThumbnail
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