Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/92711
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | T-cell Intracellular Antigen (TIA)-Proteins Deficiency in Murine Embryonic Fibroblasts Alters Cell Cycle Progression and Induces Autophagy |
Autor: | Sánchez-Jiménez, Carmen CSIC; Izquierdo, José M. CSIC ORCID | Fecha de publicación: | sep-2013 | Editor: | Public Library of Science | Citación: | PLOS One 8 (9): e75127 (2013) | Resumen: | Mice lacking either T-cell intracellular antigen 1 (TIA1) or TIA1 related/like protein (TIAR/TIAL1) show high rates of embryonic lethality, suggesting a relevant role for these proteins during embryonic development. However, intrinsic molecular and cellular consequences of either TIA1 or TIAR deficiency remain poorly defined. By using genome-wide expression profiling approach, we demonstrate that either TIA1 or TIAR inactivation broadly alter normal development-associated signalling pathways in murine embryonic fibroblasts (MEF). Indeed, these analyses highlighted alterations of cytokine-cytokine and ECM-receptor interactions and Wnt, MAPK, TGF-beta dependent signalling pathways. Consistent with these results, TIA1 and TIAR knockout (KO) MEF show reduced rates of cell proliferation, cell cycle progression delay and increased cell size. Furthermore, TIA-proteins deficiency also caused metabolic deficiencies, increased ROS levels and DNA damage, promoting a gentle rise of cell death. Concomitantly, high rates of autophagy were detected in both TIA1 and TIAR KO MEF with induction of the formation of autophagosomes, as evidenced by the up-regulation of the LC3B protein, and autolysosomes, measured by colocalization of LC3B and LAMP1, as a survival mechanism attempt. Taken together, these observations support that TIA proteins orchestrate a transcriptome programme to activate specific developmental decisions. This program is likely to contribute to mouse physiology starting at early stages of the embryonic development. TIA1/TIAR might function as cell sensors to maintain homeostasis and promote adaptation/survival responses to developmental stress. | Descripción: | 14 páginas, 8 figuras. Licencia Creative Commons Reconocimiento. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0075127 | URI: | http://hdl.handle.net/10261/92711 | DOI: | 10.1371/journal.pone.0075127 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Izquierdo _JM_PLOS_ONE_2013a.pdf | 4,34 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
27
checked on 20-abr-2024
SCOPUSTM
Citations
32
checked on 18-abr-2024
WEB OF SCIENCETM
Citations
30
checked on 28-feb-2024
Page view(s)
353
checked on 24-abr-2024
Download(s)
265
checked on 24-abr-2024