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Título

Morphologic, cytometric and functional characterization of the common octopus (Octopus vulgaris) hemocytes

Autor Castellanos-Martínez, Sheila ; Prado-Álvarez, María ; Lobo da Cunha, Alexandre; Azevedo, Carlos; Gestal, C.
Palabras clave Octopus vulgaris
Hemocytes
Flow cytometry
Functional characterization
Immune defense
Fecha de publicación 2014
EditorElsevier
Citación Developmental and Comparative Immunology 44(1): 50-58 (2014)
ResumenThe hemocytes of Octopus vulgaris were morphologically and functionally characterized. Light and electron microscopy (TEM and SEM), and flow cytometry analyses revealed the existence of two hemocyte populations. Large granulocytes showed U-shaped nucleus, a mean of 11.6 μm ± 1.2 in diameter with basophilic granules, polysaccharide and lysosomic deposits in the cytoplasm. Small granulocytes measured a mean of 8.1 μm ± 0.7 in diameter, and have a round nucleus occupying almost the entire cell and few or not granules in the cytoplasm. Flow cytometry analysis showed that large granulocytes are the principal cells that develop phagocytosis of latex beads (rising up to 56%) and ROS after zymosan stimulation. Zymosan induced the highest production of both ROS and NO. This study is the first tread towards understanding the O. vulgaris immune system by applying new tools to provide a most comprehensive morpho-functional study of their hemocytes.
Descripción 9 páginas, 7 figuras
Versión del editorhttp://dx.doi.org/10.1016/j.dci.2013.11.013
URI http://hdl.handle.net/10261/92032
DOI10.1016/j.dci.2013.11.013
ISSN0145-305X
E-ISSN1879-0089
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