English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/92020
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Generation of fatty acid chlorohydrins by adipose tissue in acute pancreatitis

AuthorsFranco-Pons, Neus ; Casas, Josefina; Fabriàs, Gemma; Gea-Sorlí, Sabrina ; Gelpí, Emili ; Closa, Daniel
Issue DateJun-2011
Citation10th World Congress on Inflammation (2011)
Abstract[Background]: Acute pancreatitis is an inflammatory process of the pancreatic gland that in the severe forms triggers the inflammation in remote organs. An additional characteristic of pancreatitis is the necrosis of peripancreatic adipose tissue due to the release of lipolytic enzymes. These areas of adipose tissue release in turn inflammatory mediators. Here we have evaluated the generation of halogenated free fatty acids by necrotic adipose tissue in a model of acute pancreatitis. [Methods]: Pancreatitis was induced in male rats by intraductal administration of 3.5% sodium taurocholate. We obtained samples of adipose tissue and ascitic fluid 3, 6 and 18 h after induction, and the levels of free fatty acids as well as fatty acid chlorohydrins were evaluated by GC–MS. In additional experiments we administered fatty acid chlorohydrins, generated by chlorination of adipose tissue lipid extracts, on the peritoneum of control animals. Three hours later we obtained peritoneal macrophages and the expression of TNFa on these cells was evaluated by RT-PCR. [Results]: During pancreatitis, necrotic areas of adipose tissue generate and release free fatty acids as well as its chlorohydrins. We identified oleic acid chlorohydrin and mono- and bis-chlorohydrin of linoleic acid in both adipose tissue and ascitic fluid. Administration of chlorinated lipids in the peritoneal cavity results in an increased expression of TNFa by peritoneal macrophages. [Conclusion]: We conclude that during severe acute pancreatitis, the necrotic areas of the peripancreatic adipose tissue generate chlorinated fatty acids. These halogenated lipids could activate macrophages and plays a role in the progression of the systemic inflammation.
DescriptionTrabajo presentado al 10th World Congress on Inflammation celebrado en Paris del 25 al 29 de junio de 2011.
URIhttp://hdl.handle.net/10261/92020
Appears in Collections:(IIBB) Comunicaciones congresos
(IQAC) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.