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The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants

AutorNocillado, J.N.; Biran, J.; Lee, Y.Y.; Levavi-Sivan, B.; Mechaly, Alejandro S. ; Zohar, Y.; Elizur, Abigail
Palabras clavePuberty
Kiss2r isoforms
Fecha de publicación15-oct-2012
CitaciónMolecular and Cellular Endocrinology 362(1-2): 211-220 (2012)
ResumenThe kisspeptin system plays an essential role in reproductive function in vertebrates, particularly in the onset of puberty. We investigated the kisspeptin system in two Perciform teleosts, the Southern Bluefin Tuna (SBT; Thunnus maccoyii), and the Yellowtail Kingfish (YTK; Seriola lalandi), by characterising their kisspeptin 2 receptor (Kiss2r) genes. In addition to the full length Kiss2r cDNA sequences, we have isolated from SBT and YTK a transcript variant that retained an intron. We have further obtained three ytk. Kiss2r transcript variants that contained deletions. In vitro functional analysis of the full length SBT and YTK Kiss2r showed higher response to Kiss2-10 than to Kiss1-10, with stronger transduction via PKC than PKA. The full length ytk. Kiss2r and two deletion variants were differentially expressed in the brain of male, but not in female, juvenile YTK treated with increasing doses of Kiss2-10 peptide. In the gonads, the expression level of the ytk. Kiss2r transcripts did not vary significantly either in the male or female fish. This is the first time that transcript variants of the Kiss2r gene that contain deletions and show responsiveness to treatments with kisspeptin have been reported in any teleost. © 2012 Elsevier Ireland Ltd.
Descripción10 pages, 9 figures, supplementary data associated with this article can be found, in the online version, athttp://dx.doi.org/10.1016/j.mce.2012.06.024
Versión del editorhttp://dx.doi.org/10.1016/j.mce.2012.06.024
Identificadoresdoi: 10.1016/j.mce.2012.06.024
issn: 0303-7207
e-issn: 1872-8057
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