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Proteomic approach to flatfish aquaculture. Study of the reproductive biology of Solea Senegalensis

Autor Forné, Ignasi
DirectorAbián, Joaquín ; Cerdà, Joan
Fecha de publicación 2007
EditorUniversidad Autónoma de Barcelona
ResumenThe flatfishes represent a group of species with a high biological and economical interest. Due to the decrease in fish hatchings, in the last years different several projects related to the aquaculture of these species were proposed. The project of this doctoral thesis has focused on the Senegal sole (Solea senegalensis), a flatfish specie with a great commercial interest and a candidate with high potential for aquaculture. However, lots of questions about its reproduction haven't been so far … [+]properly addressed, and they should be approached in order to optimize the culture of the Senegal sole. The main problems detected for this specie in captivity are the low spermiation volume and the impaired quality of spermatozoa motility males. This thesis aims to investigate the molecular mechanisms involved in the spermatogenesis and sperm production of S. senegalensis through the analysis of the testis proteome of wild and cultured animals under several conditions, in order to unveil the candidate proteins related to these processes. Initially, the proteome of S. senegalensis has been approached by de novo peptide sequencing based on mass spectrometry. Afterwards, the testis proteome from wild and cultured individuals in several spermatogenic stages was compared using two-dimensional electrophoresis and mass spectrometry. Once this point was performed, a comparative study of testis proteome from individuals cultured with and without hormonal treatment was approached. Both proteomic studies were complemented with mRNA expression analysis using microarrays. Finally, the information related to protein expression obtained during the different parts of the project, the mRNA expression and the histological analysis were integrated in a bioinformatic visual atlas specifically designed for S. senegalensis.
Descripción Doctoral thesis presented to obtain the Ph.D. in Biochemistry and Molecular Biology, Doctoral program in Biochemistry and Molecular Biology of Department of Biochemistry and Molecular Biology, Universita Autònoma de Barcelona.-- This work has been carried out in the Proteomics Laboratory CSIC/UAB and in Oryzon Genomics.
URI http://hdl.handle.net/10261/91942
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