English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/91888
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Selection of reference genes for expression studies in Cicer arietinum L.: Analysis of cyp81E3 gene expression against Ascochyta rabiei

AuthorsCastro, Patricia; Román, Belén; Rubio, Josefa; Die, José V.
KeywordsReference genes
Quantitative PCR
Ascochyta blight
Issue DateJan-2012
CitationMolecular Breeding 29(1): 261-274 (2012)
AbstractNormalisation to a reference gene is the most common method of internally controlling for error in quantitative PCR (qPCR) experiments. Studies based on qPCR in chickpea have been carried out using potential reference genes exclusively. Inappropriate normalisation may result in the acquisition of biologically irrelevant data. We have tested the expression of 12 candidate internal control genes in 36 samples representing different organs/developmental stages, genotypes and stress conditions. The most stably expressed genes were PUBQ, GAPDH, UBQ and bHLH, whereas 18S rRNA and EF-1a showed considerable regulation. The most suitable combination of reference genes for the particular experimental sets tested is provided. To illustrate the use of chickpea reference genes, we checked the expression of a putative defence gene in two different genotypes infected with Ascochyta rabiei (Pass.) Lab. The set of reference genes presented here will enable the more accurate and reliable normalisation of qPCR results for gene expression studies in this important legume crop. Our findings can be used as a starting point for reference gene selection in experimental conditions different from those tested here. © 2011 Springer Science+Business Media B.V.
Identifiersdoi: 10.1007/s11032-010-9544-8
issn: 1380-3743
e-issn: 1572-9788)
Appears in Collections:(IAS) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.