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RNA quality assessment: A view from plant qPCR studies

AuthorsDie, José V. ; Román, Belén
RNA integrity.
Issue Date8-Oct-2012
PublisherOxford University Press
CitationJournal of Experimental Botany 63(17): 6069-6077 (2012)
AbstractReverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is probably the most common molecular technique used in transcriptome analyses today. The simplicity of the technology and associated protocols that generate results without the need to understand the underlying principles has made RT-qPCR the method of choice for RNA quantification. Rather than the 'gold standard technology' often used to describe it, the performance of RT-qPCR suffers from considerable pitfalls during general workflow. The inconsistency of conventional methods for the evaluation of RNA quality and its influence on qPCR performance as well as stability of reference genes is summarized and discussed here. © 2012 The Author.
Identifiersdoi: 10.1093/jxb/ers276
issn: 0022-0957
Appears in Collections:(IAS) Artículos
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