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Title

Proteomic analysis by two-dimensional differential in gel electrophoresis (2D DIGE) of the early response of Pisum sativum to Orobanche crenata

AuthorsCastillejo Sánchez, M. Ángeles ; Fernández-Aparicio, Mónica ; Rubiales, Diego
KeywordsPisum sativum
2D DIGE
Plant response
Orobanche crenata
Mass spectrometry
Issue Date14-Sep-2012
PublisherOxford University Press
CitationJournal of Experimental Botany 63(1): 107-119 (2012)
AbstractCrenate broomrape (Orobanche crenata) is considered to be the major constraint for legume crops in Mediterranean countries. Strategies of control have been developed, but only marginal successes have been achieved. For the efficient control of the parasite, a better understanding of its interaction and associated resistance mechanisms at the molecular level is required. The pea response to this parasitic plant and the molecular basis of the resistance was studied using a proteomic approach based on 2D DIGE and MALDI-MSMS analysis. For this purpose, two genotypes showing different levels of resistance to O. crenata, as well as three time points (21, 25, and 30 d after inoculation) have been compared. Multivariate statistical analysis identified 43 differential protein spots under the experimental conditions (genotypes/treatments), 22 of which were identified using a combination of peptide mass fingerprinting (PMF) and MSMS fragmentation. Most of the proteins identified were metabolic and stress-related proteins and a high percentage of them (86%) matched with specific proteins of legume species. The behaviour pattern of the identified proteins suggests the existence of defence mechanisms operating during the early stages of infection that differed in both genotypes. Among these, several proteins were identified with protease activity which could play an important role in preventing the penetration and connection to the vascular system of the parasite. Our data are discussed and compared with those previously obtained in pea and Medicago truncatula. © 2011 The Author(s).
URIhttp://hdl.handle.net/10261/90663
DOIhttp://dx.doi.org/10.1093/jxb/err246
Identifiersdoi: 10.1093/jxb/err246
issn: 0022-0957
e-issn: 1460-2431
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