English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/8997
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

The potential benefits of insulators on heterologous constructs in transgenic animals

AutorGiraldo, Patricia; Rival-Gervier, Sylvie; Houdebine, Louis-Marie; Montoliu, Lluís
Palabras claveTransgenes
Position effects
Expression patterns
Artificial-chromosome type constructs
Heterologous genes
Insulators
Transgenic animals
Fecha de publicacióndic-2003
EditorSpringer
CitaciónTransgenic Research 12(6): 751-755 (2003)
ResumenTransgenes can display position effects, shown as variegated, ectopic, uncontrolled and even silenced expression. These undesired side effects result in irreproducible and non-systematic expression patterns in transgenic animals. The use of artificial-chromosome type constructs has been shown to overcome these position effects (Giraldo & Montoliu, 2001). However, in a large proportion of experiments with transgenic animals, constructs are made by fusing the coding region of a given gene under the control of heterologous promoter and regulatory sequences, with the aim that the expression pattern of the heterologous gene would be faithfully reproduced by the transgenic chimeric construct. In this context, the lack of endogenous regulatory sequences that are required for establishing a proper expression domain, often result in poorly controlled and suboptimally expressed transgenes (Montoliu, 2002).
DescripciónLetter to the editor.-- 5 pages, 1 table.-- PMID: 14713206 [PubMed].
Versión del editorhttp://dx.doi.org/10.1023/B:TRAG.0000005089.30408.25
URIhttp://hdl.handle.net/10261/8997
DOI10.1023/B:TRAG.0000005089.30408.25
ISSN0962-8819 (Print)
1573-9368 (Online)
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.