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Título

Immunochromatographic determination of β-lactoglobulin and its antigenic peptides in hypoallergenic formulas

Autor Puerta, Angel de la ; Díez-Masa, José Carlos; Frutos, Mercedes de
Fecha de publicación 2006
EditorElsevier
Citación International Dairy Journal 16: 406- 414 (2006)
ResumenThe use of hydrolysed formulas for feeding babies is efficient for preventing allergic diseases. Several hypoallergenic formulas (HF) based on hydrolysed cows' milk proteins have been commercialised. However, some children fed such formulas suffer allergic reactions. β-lactoglobulin (βLG) is the main allergen present in cows' milk. In addition to the whole protein, βLG fragments have also shown allergenic character. Detection of βLG and its peptides can be a useful marker of the presence of milk in food. Enzyme linked immunoassays (ELISAs) are general methods used to analyse allergens in foods. These techniques are sensitive and selective but they have several drawbacks. Immunoassay methods performed in chromatographic systems keep the advantages of classic ELISA while avoiding the disadvantages. A sandwich enzyme linked immunoaffinity chromatography (ELIAC) method was applied to determine βLG and its peptides in several commercial HF based on different milk proteins. Samples were also fractionated by employing membranes with different molecular weight cut-offs, and the antigenicity of the peptide fractions was determined. Applying the ELIAC method, for which one analysis takes 30 min, βLG was determined at the pm level in formulas based only on caseins, and at the nm to μm level in formulas based on hydrolysed whey proteins. Peptides of molecular weight less than 3000 derived from βLG showed positive results in ELIAC assays, indicating antigenic character. © 2005 Elsevier Ltd. All rights reserved.
URI http://hdl.handle.net/10261/89953
DOI10.1016/j.idairyj.2005.05.006
Identificadoresdoi: 10.1016/j.idairyj.2005.05.006
issn: 0958-6946
e-issn: 1879-0143
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