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Analysis of the HLA class I associated peptide repertoire in a hepatocellular carcinoma cell line reveals tumor-specific peptides as putative targets for immunotherapy

AuthorsÁlvarez, Iñaki; Carrascal, Montserrat ; Abián, Joaquín ; Jaraquemada, Dolores
KeywordsMass spectrometry
Issue Date2007
CitationProteomics - Clinical Applications 1(3): 286-298 (2007)
AbstractHLA class I molecules present peptides on the cell surface to CD8+ T cells. The repertoire of peptides that associate to class 1 molecules represents the cellular proteome. Therefore, cells expressing different proteomes could generate different class I-associated peptide repertoires. A large number of peptides have been sequenced from HLA class I alleles, mostly from lymphoid cells. On the other hand, T cell immunotherapy is a goal in the fight against cancer, but the identification of T cell epitopes is a laborious task. Proteomic techniques allow the definition of putative T cell epitopes by the identification of HLA natural ligands in tumor cells. In this study, we have compared the HLA class I-associated peptide repertoire from the hepatocellular carcinoma (HCC) cell line SK-Hep-1 with that previously described from lymphoid cells. The analysis ofthe peptide pool confirmed that, as expected, the peptides from SK-Hep-1 derive from proteins localized in the same compartments as in lymphoid cells. Within this pool, we have identified 12 HLA class I peptides derived from HCC-related proteins. This confirms that tumor cell lines could be a good source of tumor associated antigens to be used, together with MS, to define putative epitopes for cytotoxic T cells from cancer patients. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Identifiersdoi: 10.1002/prca.200600388
issn: 1862-8346
e-issn: 1862-8354
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