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dc.contributor.authorVialas, Vital-
dc.contributor.authorCarrascal, Montserrat-
dc.contributor.authorAbián, Joaquín-
dc.contributor.authorGil, Concha-
dc.identifier.citationJournal of Proteomics 97: 62-68 (2014)es_ES
dc.descriptionThis article is part of a Special Issue entitled: Trends in Microbial Proteomics.-- et al.es_ES
dc.description.abstractCandida albicans public proteomic datasets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22,000 distinct peptides at a 0.24% False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we attained coverage of 41% of the C. albicans open reading frame sequences (ORFs) in the database used for the searches. This PeptideAtlas provides several useful features, including comprehensive protein and peptide-centered search capabilities and visualization tools that establish a solid basis for the study of basic biological mechanisms key to virulence and pathogenesis such as dimorphism, adherence, and apoptosis. Further, it is a valuable resource for the selection of candidate proteotypic peptides for targeted proteomic experiments via Selected Reaction Monitoring (SRM) or SWATH-MS. [Biological significance]: This C. albicans PeptideAtlas resolves the previous absence of fungal pathogens in the PeptideAtlas project. It represents the most extensive characterization of the proteome of this fungus that exists up to the current date, including evidence for uncharacterized ORFs. Through its web interface, PeptideAtlas supports the study of interesting proteins related to basic biological mechanisms key to virulence such as apoptosis, dimorphism and adherence. It also provides a valuable resource to select candidate proteotypic peptides for future (SRM) targeted proteomic experiments.es_ES
dc.description.sponsorshipThis work was supported by BIO 2009-07654 and BIO 2012-31767 from the Ministerio de Economía y Competitividad, PROMPT (S2010/BMD-2414) from the Comunidad de Madrid, and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD12/0015) -co-financed by the European Development Regional Fund “A way to achieve Europe” ERDF. EWD, ZS, and RLM are supported in part by the National Institute of General Medical Sciences, under Grant No. R01 GM087221, 2P50 GM076547/Center for Systems Biology, the National Science Foundation MRI [Grant No. 0923536], the EU FP7 grant ‘ProteomeXchange’ [Grant No. 260558], and by the Luxembourg Centre for Systems Biomedicine and the University of Luxembourg. RA is supported in part by ERC advanced grant ‘Proteomics v3.0’ (Grant No. 233226) of the European Uniones_ES
dc.titleA Candida albicans PeptideAtlases_ES
dc.description.peerreviewedPeer reviewedes_ES
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