English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/89287
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Isotope dilution mass spectrometry for absolute quantification in proteomics: Concepts and strategies

AutorVillanueva, Joan ; Carrascal, Montserrat ; Abián, Joaquín
Palabras claveAbsolute protein quantification
Quality control
Internal standard
Quantitative proteomics
Fecha de publicación2014
EditorElsevier
CitaciónJournal of Proteomics 96: 184-199 (2014)
ResumenIsotope dilution mass spectrometry is a reference technique for quantitative analysis, given that it combines the sensitivity and selectivity of MS instruments with the precision and accuracy associated with the use of internal standards. Isotope-labeled proteins are the optimal internal standards for quantitative proteomics as they closely mimic the behavior of their natural counterparts during the analytical process. A major complication of isotope dilution mass spectrometry proteomics is the technical difficulty of obtaining these internal standards, especially in studies where a high number of proteins have to be quantified simultaneously. In this paper, we review some of the characteristics of the isotope dilution mass spectrometry approach, its benefits in terms of reliability and quality control in targeted proteomic analysis and the different strategies developed for its application in proteomics.© 2013 Elsevier B.V.
URIhttp://hdl.handle.net/10261/89287
DOI10.1016/j.jprot.2013.11.004
Identificadoresdoi: 10.1016/j.jprot.2013.11.004
issn: 1874-3919
e-issn: 1876-7737
Aparece en las colecciones: (IIBB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.