English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/89019
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Culturing Bias in Marine Heterotrophic Flagellates Analyzed Through Seawater Enrichment Incubations

AuthorsCampo, Javier del ; Balagué, Vanessa ; Forn, Irene ; Lekunberri, Itziar ; Massana, Ramon
Issue DateOct-2013
CitationMicrobial Ecology 66(3): 489-499 (2013)
AbstractThe diversity of heterotrophic flagellates is generally based on cultivated strains, on which ultrastructural, physiological, and molecular studies have been performed. However, the relevance of these cultured strains as models of the dominant heterotrophic flagellates in the marine planktonic environment is unclear. In fact, molecular surveys typically recover novel eukaryotic lineages that have refused cultivation so far. This study was designed to directly address the culturing bias in planktonic marine heterotrophic flagellates. Several microcosms were established adding increasing amounts and sources of organic matter to a confined natural microbial community pre-filtered by 3 μm. Growth dynamics were followed by epifluorescence microscopy and showed the expected higher yield of bacteria and heterotrophic flagellates at increased organic matter additions. Moreover, protist diversity analyzed by molecular tools showed a clear substitution in the community, which differed more and more from the initial sample as the organic matter increased. Within this gradient, there was also an increase of sequences related to cultured organisms as well as a decrease in diversity. Culturing bias is partly explained by the use of organic matter in the isolation process, which drives a shift in the community to conditions closer to laboratory cultures. An intensive culturing effort using alternative isolation methods is necessary to allow the access to the missing heterotrophic flagellates that constitute the abundant and active taxa in marine systems. © 2013 Springer Science+Business Media New York
Description11 pages, 6 figures, 2 tables, supplementary material in https://doi.org/10.1007/s00248-013-0251-y
Publisher version (URL)https://doi.org/10.1007/s00248-013-0251-y
Identifiersdoi: 10.1007/s00248-013-0251-y
issn: 0095-3628
e-issn: 1432-184X
Appears in Collections:(ICM) Artículos
(IBE) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.