English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/88957
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

The transmembrane domain of the T4SS coupling protein TrwB and its role in protein-protein interactions

AuthorsSegura, Rosa L.; Águila, Sandra; Ugarte-Uribe, Begoña; Vecino, Ana J.; Cruz, Fernando de la ; Goñi, Félix M.; Alkorta, Itziar
KeywordsConjugative plasmid
VirB10-like proteins
Coupling protein
Type IV secretion system
Bacterial conjugation
Issue Date2013
PublisherElsevier
CitationBBA - Biomembranes 1828(9): 2015-2025 (2013)
AbstractBacteria use type IV secretion systems to transfer genetic material and proteins from donor to recipient cells, using proteins encoded by conjugative plasmids. Among those proteins the so-called Type IV Coupling Protein plays a central role in the process. One of the best studied members of this family is TrwB, the conjugative coupling protein of R388 plasmid. Previous studies indicated that the transmembrane domain of TrwB plays a role beyond the mere anchoring of the protein to the membrane. TrwB has also been shown to interact with other conjugative proteins, such as the VirB10-like protein of R388 TrwE. The goal of this study is to elucidate the role of the different domains of TrwB and TrwE in their biological function, and in both self- and TrwB-TrwE interactions. To this aim, a series of TrwB and TrwE deletion mutant proteins were constructed. Conjugation and interaction studies revealed that the transmembrane domain of TrwB, and particularly its second transmembrane helix, is needed for TrwB self-interaction and for R388 conjugative transfer and that there are contacts between TrwB and TrwE in the membrane. On the contrary, the lack of the TMD of TrwE does not completely abolish R388 conjugation although the interaction between TrwE-TrwB is lost. These results identify protein-protein interactions inside the membrane needed for T4SS function. © 2013 Elsevier B.V.
URIhttp://hdl.handle.net/10261/88957
DOI10.1016/j.bbamem.2013.05.022
Identifiersdoi: 10.1016/j.bbamem.2013.05.022
issn: 0005-2736
Appears in Collections:(IBBTEC) Artículos
(UBF) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.