English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/88722
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Reverse-engineering post-transcriptional regulation of gap genes in Drosophila melanogaster

AuthorsBecker, Kolja; Balsa-Canto, Eva ; Cicin-Sain, Damjan; Hoermann, Astrid; Janssens, Hilde; Banga, Julio R. ; Jaeger, Johannes
Issue Date2013
PublisherPublic Library of Science
CitationPLoS Computational Biology 9(10): e1003281 (2013)
AbstractSystems biology proceeds through repeated cycles of experiment and modeling. One way to implement this is reverse engineering, where models are fit to data to infer and analyse regulatory mechanisms. This requires rigorous methods to determine whether model parameters can be properly identified. Applying such methods in a complex biological context remains challenging. We use reverse engineering to study post-transcriptional regulation in pattern formation. As a case study, we analyse expression of the gap genes Krüppel, knirps, and giant in Drosophila melanogaster. We use detailed, quantitative datasets of gap gene mRNA and protein expression to solve and fit a model of post-transcriptional regulation, and establish its structural and practical identifiability. Our results demonstrate that post-transcriptional regulation is not required for patterning in this system, but is necessary for proper control of protein levels. Our work demonstrates that the uniqueness and specificity of a fitted model can be rigorously determined in the context of spatio-temporal pattern formation. This greatly increases the potential of reverse engineering for the study of development and other, similarly complex, biological processes
Description16 páginas, 6 figuras, 1 tabla
Publisher version (URL)http://dx.doi.org/10.1371/journal.pcbi.1003281
URIhttp://hdl.handle.net/10261/88722
DOIhttp://dx.doi.org/10.1371/journal.pcbi.1003281
ISSN1553-734X
E-ISSN1553-7358
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Reverse_engineering_Drosophila.pdf1,69 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.