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Wide-field time-resolved fluorescence anisotropy imaging (TR-FAIM): Imaging the rotational mobility of a fluorophore

AuthorsSiegel, Jan ; Suhling, Klaus; Lévêque Fort, Sandrine; Webb, Stephen E.D.; Davis, Daniel M.; Phillips, David; Sabharwal, Y.; French, Paul M. W.
Issue Date2003
PublisherAmerican Institute of Physics
CitationReview of Scientific Instruments 74: 182-192 (2003)
AbstractA picosecond time-gated fluorescence lifetime imaging (FLIM) system extended to perform time-resolved fluorescence anisotropy imaging (TR-FAIM) was reported. The imaging technique presented reports the rotational mobility of a fluorophore as it varies according to the local environment. Wide-field maps of rotational correlation time and viscosity were obtained which illustrated the potential to image the local viscosity and fluorescence lifetime distributions of fluorophore tagged proteins in cells. © 2003 American Institute of Physics
Identifiersdoi: 10.1063/1.1519934
issn: 0034-6748
Appears in Collections:(CFMAC-IO) Artículos
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