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http://hdl.handle.net/10261/88379
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Zaouali, Mohamed A. | - |
dc.contributor.author | Boncompagni, Eleonora | - |
dc.contributor.author | Bejaoui, Mohamed | - |
dc.contributor.author | Pantazi, Eirini | - |
dc.contributor.author | Folch-Puy, Emma | - |
dc.contributor.author | Roselló-Catafau, Joan | - |
dc.date.accessioned | 2013-12-10T14:05:41Z | - |
dc.date.available | 2013-12-10T14:05:41Z | - |
dc.date.issued | 2013 | - |
dc.identifier | doi: 10.1111/jpi.12051 | - |
dc.identifier | issn: 0742-3098 | - |
dc.identifier | e-issn: 1600-079X | - |
dc.identifier.citation | Journal of Pineal Research 55(1): 65-78 (2013) | - |
dc.identifier.uri | http://hdl.handle.net/10261/88379 | - |
dc.description.abstract | Ischemia/reperfusion injury (IRI) associated with liver transplantation plays an important role in the induction of graft injury. Prolonged cold storage remains a risk factor for liver graft outcome, especially when steatosis is present. Steatotic livers exhibit exacerbated endoplasmic reticulum (ER) stress that occurs in response to cold IRI. In addition, a defective liver autophagy correlates well with liver damage. Here, we evaluated the combined effect of melatonin and trimetazidine as additives to IGL-1 solution in the modulation of ER stress and autophagy in steatotic liver grafts through activation of AMPK. Steatotic livers were preserved for 24 hr (4°C) in UW or IGL-1 solutions with or without MEL + TMZ and subjected to 2-hr reperfusion (37°C). We assessed hepatic injury (ALT and AST) and function (bile production). We evaluated ER stress (GRP78, PERK, and CHOP) and autophagy (beclin-1, ATG7, LC3B, and P62). Steatotic livers preserved in IGL-1 + MEL + TMZ showed lower injury and better function as compared to those preserved in IGL-1 alone. IGL-1 + MEL + TMZ induced a significant decrease in GRP78, pPERK, and CHOP activation after reperfusion. This was consistent with a major activation of autophagic parameters (beclin-1, ATG7, and LC3B) and AMPK phosphorylation. The inhibition of AMPK induced an increase in ER stress and a significant reduction in autophagy. These data confirm the close relationship between AMPK activation and ER stress and autophagy after cold IRI. The addition of melatonin and TMZ to IGL-1 solution improved steatotic liver graft preservation through AMPK activation, which reduces ER stress and increases autophagy. © 2012 John Wiley & Sons A/S. | - |
dc.description.sponsorship | This work was supported by FIS grant PI081988 to Joan Rosello-Catafau from the Spanish Ministry of Health. Mohamed Bejaoui is a fellowship holder from CSIC (ICOOP 0005 project) and Eirini Pantazi is the recipient of an AGAUR fellow (Generalitat de Catalunya, Barcelona). | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-Blackwell | - |
dc.rights | closedAccess | - |
dc.subject | IGL-1 preservation solution | - |
dc.subject | ER stress | - |
dc.subject | Cold ischemia reperfusion injury | - |
dc.subject | Autophagy | - |
dc.subject | AMPK | - |
dc.title | AMPK involvement in endoplasmic reticulum stress and autophagy modulation after fatty liver graft preservation: a role for melatonin and trimetazidine cocktail | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1111/jpi.12051 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1111/jpi.12051 | - |
dc.date.updated | 2013-12-10T14:05:41Z | - |
dc.description.version | Peer Reviewed | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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