English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/8834
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Differential CD4+ versus CD8+ T-Cell Responses Elicited by Different Poxvirus-Based Human Immunodeficiency Virus Type 1 Vaccine Candidates Provide Comparable Efficacies in Primates

AuthorsMooij, Petra; Balla-Jhagjhoorsingh, Sunita S.; Koopman, Gerrit; Beenhakker, Niels; Van Haaften, Patricia; Baak, Ilona; Nieuwenhuis, Ivonne G.; Kondova, Ivanela; Wagner, Ralf; Wolf, Hans; Gómez, Carmen E.; Nájera García, José Luis; Jiménez, Victoria; Esteban, Mariano; Heeney, Jonathan L.
KeywordsImmune response enhancement
Poxvirus vectors
Human immunodeficiency virus (HIV)
Vaccine candidates
New York vaccinia virus (NYVAC)
Modified vaccinia virus Ankara (MVA)
CD4+/CD8+ T-cell immune response
Indian rhesus macaques
Issue Date9-Jan-2008
PublisherAmerican Society for Microbiology
CitationJournal of Virology 82(6): 2975-2988 (2008)
AbstractPoxvirus vectors have proven to be highly effective for boosting immune responses in diverse vaccine settings. Recent reports reveal marked differences in the gene expression of human dendritic cells infected with two leading poxvirus-based human immunodeficiency virus (HIV) vaccine candidates, New York vaccinia virus (NYVAC) and modified vaccinia virus Ankara (MVA). To understand how complex genomic changes in these two vaccine vectors translate into antigen-specific systemic immune responses, we undertook a head-to-head vaccine immunogenicity and efficacy study in the pathogenic HIV type 1 (HIV-1) model of AIDS in Indian rhesus macaques. Differences in the immune responses in outbred animals were not distinguished by enzyme-linked immunospot assays, but differences were distinguished by multiparameter fluorescence-activated cell sorter analysis, revealing a difference between the number of animals with both CD4+ and CD8+ T-cell responses to vaccine inserts (MVA) and those that elicit a dominant CD4+ T-cell response (NYVAC). Remarkably, vector-induced differences in CD4+/CD8+ T-cell immune responses persisted for more than a year after challenge and even accompanied antigenic modulation throughout the control of chronic infection. Importantly, strong preexposure HIV-1/simian immunodeficiency virus-specific CD4+ T-cell responses did not prove deleterious with respect to accelerated disease progression. In contrast, in this setting, animals with strong vaccine-induced polyfunctional CD4+ T-cell responses showed efficacies similar to those with stronger CD8+ T-cell responses.
Description14 pages, 8 figures.-- PMID: 18184713 [PubMed].-- Supplementary material available: Table S1: DNA sequence of MVA-89.6p-SIVgpn in the TK viral locus.-- Printed version published on Mar 2008.
Publisher version (URL)http://dx.doi.org/10.1128/JVI.02216-07
URIhttp://hdl.handle.net/10261/8834
DOI10.1128/JVI.02216-07
ISSN0022-538X
Appears in Collections:(CNB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.