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Regeneration of virus-free plants by in vitro chemotherapy of GFLV (Grapevine Fanleaf Virus) infected explants of Vitis vinifera L. cv "Zalema"

AuthorsWeiland, Carlos; Cantos, Manuel ; Troncoso de Arce, Antonio ; Pérez Camacho, Fernando
Vitis vinifera
Issue DateJul-2004
PublisherInternational Society for Horticultural Science
CitationActa Horticulturae 652: 463-466 (2004)
AbstractIn vitro chemotherapy using ribavirin or acyclovir was applied to eliminate Grapevine fanleaf virus (GFLV) from Vitis vinifera cv 'Zalema' plant material. Ribavirin at 20 mgl-1 of concentration eliminated GFLV from the 94% of the grapevine in vitro shoots, enabling GFLV virus free plants to be obtained. The ribavirin treatment did not affect plant growth, rooting and number of roots, and did not cause chlorosis, hyperhydration or apex necrosis. Acyclovir at the same concentration was ineffective in regenerating GFLV-free plants and clearly negatively affected the explant development producing apical necrosis.
Description4 páginas, 1 tabla, 24 referencias.-- I International Symposium on Grapevine Growing, Commerce and Research, celebrado del 30 de junio-2 de julio 2003, en Lisboa, Portugal.
Appears in Collections:(IRNAS) Artículos
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