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A simple and effective calibration method to determine the accuracy of liquid-handling nano-dispenser devices

AuthorsRodríguez-Puente, Sonia; Linacero-Blanco, Judith; Guasch, Alicia
Liquid-handling nano-dispenser devices
Issue Date2013
PublisherInternational Union of Crystallography
CitationActa Crystallographica Section F 69(3): 336-341 (2013)
AbstractThe accurate delivery of small volumes is a critical factor in the crystallization of macromolecules as it influences the reproducibility of the screening experiments. Crystallographic screening technologies have made it possible to perform experiments using volumes as low as 50nl. The accuracy of the dispenser has usually been calibrated by weight measurements. In this work, a simple and inexpensive fluorescence-based calibration method that is sensitive and that can be used to monitor the precision and accuracy of any liquid-handling nano-dispenser device is presented. The results suggest that the protocol described here can be useful to determine volumes ranging from 50 to 300nl with precision. Therefore, the pipetting of volumes as low as 50nl can be calibrated periodically to ensure that precision and accuracy are maintained. The suggested calibration protocol can be executed in 6h per instrument, including the calibration curve, which is the most time-consuming step; the rest can be completed in approximately 2h. © 2013 International Union of Crystallography All rights reserved.
Publisher version (URL)http://dx.doi.org/10.1107/S1744309113000791
Identifiersdoi: 10.1107/S1744309113000791
issn: 1744-3091
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