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Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces

AuthorsHofmann, Kerstin; Silvar Casao, Cristina ; Casas Cendoya, Ana María ; Herz, Markus; Büttner, Bianca; Gracia Gimeno, María Pilar ; Contreras-Moreira, Bruno ; Wallwork, Hugh; Igartua Arregui, Ernesto ; Schweizer, Günther
Issue DateDec-2013
CitationHoffmann K, Silvar C, Casas AM, Herz M, Büttner B, Gracia MP, Contreras-Moreira B, Wallwork H, Igartua E, Schweizer G. Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces. Theoretical and Applied Genetics 126 (12): 3091-3102 (2013)
AbstractScald, caused by Rhynchosporium commune, is one of the most prevalent barley diseases worldwide. A search for new resistance sources revealed that Spanish landrace-derived lines SBCC145 and SBCC154 showed outstanding resistance to scald. They were crossed to susceptible cultivar Beatrix to create large DH-mapping populations of 522 and 416 DH lines that were scored for disease resistance in the greenhouse using two R. commune isolates. To ascertain the pattern of resistance, parents and reference barley lines with known scald resistance were phenotyped with a panel of differential R. commune isolates. Subpopulations were genotyped with the Illumina GoldenGate 1,536 SNP Assay and a large QTL in the centromeric region of chromosome 3H, known to harbour several scald resistance genes and/or alleles, was found in both populations. Five SNP markers closest to the QTL were converted into CAPS markers. These CAPS markers, together with informative SSR markers used in other scald studies, confirmed the presence of the Rrs1 locus. The panel of differential scald isolates indicated that the allele carried by both donors was Rrs1 Rh4 . The genetic distance between Rrs1 and its flanking markers was 1.2 cM (11_0010) proximally and 0.9 cM (11_0823) distally, which corresponds to a distance of just below 9 Mbp. The number and nature of scald resistance genes on chromosome 3H are discussed. The effective Rrs1 allele found and the closely linked markers developed are already useful tools for molecular breeding programs and provide a good step towards the identification of candidate genes.
Description25 Pags., 5 Tabls., 2 Figs. The definitive version, with all the supplementary materials, is available at: http://link.springer.com/journal/122
Publisher version (URL)http://dx.doi.org/10.1007/s00122-013-2196-4
Appears in Collections:(EEAD) Artículos
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