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Título

Nucleolar exit of RNF8 and BRCA1 in response to DNA damage

Autor Guerra-Rebollo, Marta; Mateo, Francesca ; Framke, Kristin ; Huen, Michael S. Y.; Plans, Vanessa; Thomson, Timothy M.
Palabras clave DNA damage
BRCA1
RNF8
Nucleolus
Fecha de publicación 2012
EditorElsevier
Citación Experimental Cell Research 318(18): 2365-2376 (2012)
ResumenThe induction of DNA double-strand breaks (DSBs) elicits a plethora of responses that redirect many cellular functions to the vital task of repairing the injury, collectively known as the DNA damage response (DDR). We have found that, in the absence of DNA damage, the DSB repair factors RNF8 and BRCA1 are associated with the nucleolus. Shortly after exposure of cells to γ-radiation, RNF8 and BRCA1 translocated from the nucleolus to damage foci, a traffic that was reverted several hours after the damage. RNF8 interacted through its FHA domain with the ribosomal protein RPSA, and knockdown of RPSA caused a depletion of nucleolar RNF8 and BRCA1, suggesting that the interaction of RNF8 with RPSA is critical for the nucleolar localization of these DDR factors. Knockdown of RPSA or RNF8 impaired bulk protein translation, as did γ-irradiation, the latter being partially countered by overexpression of exogenous RNF8. Our results suggest that RNF8 and BRCA1 are anchored to the nucleolus through reversible interactions with RPSA and that, in addition to its known functions in DDR, RNF8 may play a role in protein synthesis, possibly linking the nucleolar exit of this factor to the attenuation of protein synthesis in response to DNA damage. © 2012 Elsevier Inc.
URI http://hdl.handle.net/10261/87941
DOI10.1016/j.yexcr.2012.07.003
Identificadoresdoi: 10.1016/j.yexcr.2012.07.003
issn: 0014-4827
e-issn: 1090-2422
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