English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/87941
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Nucleolar exit of RNF8 and BRCA1 in response to DNA damage

AutorGuerra-Rebollo, Marta; Mateo, Francesca ; Framke, Kristin ; Huen, Michael S. Y.; Plans, Vanessa; Thomson, Timothy M.
Palabras claveDNA damage
BRCA1
RNF8
Nucleolus
Fecha de publicación2012
EditorElsevier
CitaciónExperimental Cell Research 318(18): 2365-2376 (2012)
ResumenThe induction of DNA double-strand breaks (DSBs) elicits a plethora of responses that redirect many cellular functions to the vital task of repairing the injury, collectively known as the DNA damage response (DDR). We have found that, in the absence of DNA damage, the DSB repair factors RNF8 and BRCA1 are associated with the nucleolus. Shortly after exposure of cells to γ-radiation, RNF8 and BRCA1 translocated from the nucleolus to damage foci, a traffic that was reverted several hours after the damage. RNF8 interacted through its FHA domain with the ribosomal protein RPSA, and knockdown of RPSA caused a depletion of nucleolar RNF8 and BRCA1, suggesting that the interaction of RNF8 with RPSA is critical for the nucleolar localization of these DDR factors. Knockdown of RPSA or RNF8 impaired bulk protein translation, as did γ-irradiation, the latter being partially countered by overexpression of exogenous RNF8. Our results suggest that RNF8 and BRCA1 are anchored to the nucleolus through reversible interactions with RPSA and that, in addition to its known functions in DDR, RNF8 may play a role in protein synthesis, possibly linking the nucleolar exit of this factor to the attenuation of protein synthesis in response to DNA damage. © 2012 Elsevier Inc.
URIhttp://hdl.handle.net/10261/87941
DOI10.1016/j.yexcr.2012.07.003
Identificadoresdoi: 10.1016/j.yexcr.2012.07.003
issn: 0014-4827
e-issn: 1090-2422
Aparece en las colecciones: (IBMB) Artículos
(CIC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.