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Título

NMR studies on carbohydrate interactions with DC-SIGN towards a quantitative STD analysis

Autor Guzzi, Cinzia ; Muñoz-García, Juan Carlos; Enríquez-Navas, Pedro Miguel; Rojo, Javier ; Angulo, Jesús ; Nieto, Pedro M.
Fecha de publicación 2013
EditorInternational Union of Pure and Applied Chemistry
Citación Pure and Applied Chemistry 85: 1771- 1787 (2013)
ResumenThe recent introduction of saturation transfer difference (STD) NMR has increased the tools for the study of protein-carbohydrate complexes. This is useful when it is combined with transfer nuclear Overhauser enhancement spectroscopy (NOESY) measurement, or when it is interpreted using the expected calculated values of transference, yielding additional, very valuable information for the study of this type of complex. The objective of this work is to cover the advances of the STD technique as exemplified by the investigations of DC-SIGN (dendritic cell-specific ICAM-3 grabbing non-inte-grin) recognition by simple carbohydrates or mimics of them, based on structures containing a terminal mannose or fucose. We also will discuss the methods for quantification of the STD values based on the initial growing rates with the saturation time. © 2013 IUPAC.
URI http://hdl.handle.net/10261/87356
DOI10.1351/PAC-CON-12-11-14
Identificadoresdoi: 10.1351/PAC-CON-12-11-14
issn: 0033-4545
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