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Title

A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein

AuthorsJulve, José Manuel ; Gandía, Antoni; Fernández-del-Carmen, Asun ; Sarrion-Perdigones, Alejandro ; Castelijns, Bas; Granell, Antonio ; Orzáez, Diego
Issue Date2013
PublisherSpringer
CitationPlant Molecular Biology 81(6): 553-564 (2013)
AbstractNew evidence is emerging which indicates that population variants in plant virus infections are not uniformly distributed along the plant, but structured in a mosaic-like pattern due to limitation to the superinfection imposed by resident viral clones. The mechanisms that prevent the infection of a challenge virus into a previously infected cell, a phenomenon known as superinfection exclusion (SE) or Homologous Interference, are only partially understood. By taking advantage of a deconstructed tobacco mosaic virus (TMV) system, where the capsid protein (CP) gene is replaced by fluorescent proteins, an exclusion mechanism independent of CP was unveiled. Time-course superinfection experiments provided insights into SE dynamics. Initial infection levels affecting less than 10 % of cells led to full immunization in only 48 h, and measurable immunization levels were detected as early as 6 h post-primary infection. Depletion of a functional movement protein (MP) was also seen to slow down, but not to prevent, the SE mechanism. These observations suggest a CP-independent mechanism based on competition for a host-limiting factor, which operates at very low virus concentration. The possible involvement of host factors in SE has interesting implications as it would enable the host to influence the process. © 2013 Springer Science+Business Media Dordrecht.
URIhttp://hdl.handle.net/10261/87166
DOI10.1007/s11103-013-0028-1
Identifiersdoi: 10.1007/s11103-013-0028-1
issn: 0167-4412
e-issn: 1573-5028
Appears in Collections:(IBMCP) Artículos
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