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Title

XMIPP: a new generation of an open-source image processing package for electron microscopy

AuthorsSánchez Sorzano, Carlos Óscar; Marabini, Roberto; Velázquez-Muriel, Javier; Bilbao-Castro, José Román; Scheres, Sjors H. W.; Carazo, José M.; Pascual-Montano, Alberto
KeywordsElectron microscopy
Single particles
Software packages
Image processing
3D reconstruction
Issue Date14-Aug-2004
PublisherElsevier
CitationJournal of Structural Biology 148(2): 194-204 (2004)
AbstractX-windows based microscopy image processing package (Xmipp) is a specialized suit of image processing programs, primarily aimed at obtaining the 3D reconstruction of biological specimens from large sets of projection images acquired by transmission electron microscopy. This public-domain software package was introduced to the electron microscopy field eight years ago, and since then it has changed drastically. New methodologies for the analysis of single-particle projection images have been added to classification, contrast transfer function correction, angular assignment, 3D reconstruction, reconstruction of crystals, etc. In addition, the package has been extended with functionalities for 2D crystal and electron tomography data. Furthermore, its current implementation in C++, with a highly modular design of well-documented data structures and functions, offers a convenient environment for the development of novel algorithms. In this paper, we present a general overview of a new generation of Xmipp that has been re-engineered to maximize flexibility and modularity, potentially facilitating its integration in future standardization efforts in the field. Moreover, by focusing on those developments that distinguish Xmipp from other packages available, we illustrate its added value to the electron microscopy community.
Description11 pages, 2 figures.-- PMID: 15477099 [PubMed].-- Printed version published on Nov 2004.
Publisher version (URL)http://dx.doi.org/10.1016/j.jsb.2004.06.006
URIhttp://hdl.handle.net/10261/8706
DOI10.1016/j.jsb.2004.06.006
ISSN1047-8477
Appears in Collections:(CNB) Artículos
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