English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/86831
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Actin expression in germinating seeds of Phaseolus vulgaris L.

AuthorsVillanueva, Marco A.; Campos, Francisco ; Díaz, Claudia; Colmenero Flores, José M. ; Dantán, E.; Sánchez, Federico; Covarrubias, A. A.
Issue Date1999
CitationPlanta 207 (4): 582-589 (1999)
AbstractActin was present at very low levels in the seeds of common bean (Phaseolus vulgaris L.) compared with those from other species, and was observed mostly in the embryo. A time-course of actin expression in germinating bean seeds revealed an induced expression of both the mRNA and protein. Initially, the actin mRNA in seeds was barely detectable by northern blot analysis. However, there was a substantial increase in the expression of the actin mRNA at 24, 48 and 72 h after imbibition, compared with an internal control consisting of a late-embryogenesis-abundant (LEA) type IV gene from P. vulgaris. An increase in the amount of actin in total seed extracts that parallelled that of the mRNA was detected by western blotting starting at 24 h after imbibition. This increase was more apparent when the embryo alone was analyzed. Two-dimensional western blots initially revealed three actin isoforms with isoelectric points (pIs) of approximately 5.6, 5.7 and 5.8, the amounts of which increased within a 48-h period, when a new minor isoform of pI approximately 5.5 appeared; however, after 72 h, the pI-5.8 isoform had almost disappeared and the pI-5.5 isoform had disappeared completely, indicating that these two minor isoforms are expressed transiently. These results indicate that actin is at very low levels in the dry seed but undergoes an increased and differential expression during imbibition, an event probably required to carry out all the necessary functions for germination.
Publisher version (URL)http://dx.doi.org/10.1007/s004250050521
Identifiersdoi: 10.1007/s004250050521
issn: 0032-0935
e-issn: 1432-2048
Appears in Collections:(IRNAS) Artículos
Files in This Item:
File Description SizeFormat 
Actin expression.pdf1,96 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.