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dc.contributor.authorMartín-Hernández, Ana M.-
dc.contributor.authorGutiérrez-Rivas, Mónica-
dc.contributor.authorDomingo, Esteban-
dc.contributor.authorMenéndez-Arias, Luis-
dc.identifier.citationNucleic Acids Res., 1997; 25: 1383 - 1389.en_US
dc.description.abstractThe role of Tyr115 of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) in the mispair extension fidelity of DNA dependent DNA synthesis was analysed by using a series of 15 mutant enzymes with substitutions at Tyr115. Their kinetic parameters for elongation using homopolymeric RNA-DNA and heteropolymeric DNA-DNA complexes showed major effects of the amino acid substitutions on the K m value for dNTP. Enzymes with large hydrophobic residues at position 115 displayed lower K m values than enzymes with small and charged amino acids at this position. The influence of all these amino acid replacements in mispair extension fidelity assays was analyzed using three different mismatches (A:C, A:G and A:A) at the 3 ' -terminal position of the primer DNA. For the A:C mispair, a 2.6-33.4-fold increase in mispair extension efficiency ( f ext) was observed as compared with the wild-type enzyme. Unexpectedly, all the mutants tested as well as the wild-type RT were very efficient in extending the A:G and A:A transversion mispairs. This effect was due to the template-primer sequence context and not to the buffer conditions of the assay. The data support a role of Tyr115 in accommodating the complementary nucleotide into the nascent DNA while polymerization takes place.en_US
dc.description.sponsorshipThis work was supported by grants from Fondo de Investigaciones Sanitarias (95/0034-1), Comunidad Autónoma de Madrid, Fundación Ramón Areces, and Fundación Rodríguez Pascual.en_US
dc.format.extent96005 bytes-
dc.publisherOxford University Pressen_US
dc.subjectHIV-1 RTen_US
dc.titleMispair extension fidelity of human immunodeficiency virus type 1 reverse transcriptases with amino acid substitutions affecting Tyr115en_US
dc.description.peerreviewedPeer revieweden_US
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderComunidad de Madrid-
dc.contributor.funderFundación Ramón Areces-
dc.contributor.funderFundación Eugenio Rodríguez Pascual-
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