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Use of Electrical Impedance Spectroscopy (EIS) to monitor cryoprotectant concentration in cellular and tissue cryopreservation protocols

AuthorsOlmo, Alberto; Yúfera, A.
Issue Date2010
CitationCryobiology 61(3): 392 (2010)
AbstractIn this paper we theoretically analyse the use of Electrical Impedance Spectroscopy (EIS) to efficiently monitor cryoprotectant concentration in cryopreservation protocols. In order to correctly determine the perfusion of cryoprotectant inside tissues and organs, with its spatial distribution, it is necessary to previously study the influence that the frequency, temperature and electrode configuration have in bioimpedance measurements. We have analysed with COMSOL Multiphysics software the frequency response of a 2-electrode system to different concentrations of Me2SO, perfused into 3T3 fibroblasts and monolayers of Mesenchymal Stem Cells (MSCs), fundamental for tissue-based therapeutics. An electrical model based on a previous work was used (Olmo et al., 2010). This model constitutes a complete electrical description of the electrode-cell system, including the electrode-electrolyte double layer, electrode-cell gap, electrolyte-cell double layer and cellular membrane, besides taking into account electrical properties of intra and extra-cellular medium. The Quasi-statics module of COMSOL was used to perform the finite element simulations, for different frequencies. Simulations show the system easily detects changes in cryoprotectant concentration, being necessary to optimize both frequency and electrode configuration to efficiently detect cryoprotectant perfusion inside the biological material. Temperature influence on conductivity and permittivity, as well as on electrode interfaces, has also been discussed in detail. The model and finite element method simulation herewith reported has proved to be a good tool, which can be used to optimize the design of experimental setups. These models can also be extended in the future, to analyse the use of more complex three-dimensional EIS systems, which can monitor cryoprotectant perfusion in the cryopreservation of more complex tissues and organs.
DescriptionTrabajo presentado al 47TH Annual Meeting on the Society for Cryobiology (CRYO-2010) celebrado en Bristol (UK).
Appears in Collections:(IMSE-CNM) Artículos
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